英：
Person’s Elongator compound which separates from the HeLa cell is composing and with RNAPⅡIn the function way and yeast’s Elongator compound is very similar, but are extremely few to its function research. In order to study person’s Elongator compound to catalyze Asian base Elp3 the function, genes and so on person elp3 changes over to the yeast elp3 gene flaw the sudden change strain (elp3△ strain), and carries on the function supplementary experiment and ssa3 and the pho5 gene expression analysis to the transformed strain, finally indicated that the person elp3 gene may obviously restore the sudden change strain to high temperature and the Caffeine sensitivity, has obviously compensated the flaw which under the low phosphorus condition the sudden change pho5 gene expression retards, and may stir up under the condition to enhance the ssa3 gene hotly the expression. Area and person elp3 HAT area fusion yhelp3 has the stronger compensation ability including yeast elp3 the non-HAT to the above flaw. But HAT area catalysis structure territory flaw’s yhelp3HAT- any compensation ability, had not indicated that the person Elp3 Asia base is possible and yeast’s this Asian base function is similar, the person Elp3 HAT activeness also exercises the function for it to.

译：

从HeLa细胞中分离的人的Elongator复合物在组成及与RNAPⅡ的作用方式上与酵母的Elongator复合物十分相似，但对其功能研究极少。为了研究人的Elongator复合物催化亚基Elp3的功能，将人elp3等基因转入酵母elp3基因缺失的突变菌株(elp3△菌株)，并对转化菌株进行功能互补实验和ssa3和pho5基因表达分析，结果表明人elp3基因可显著恢复突变菌株对高温和Caffeine的敏感性，在低磷条件下显著补偿了突变株pho5基因表达延迟的缺陷，并可在热激条件下提高ssa3基因的表达。含酵母elp3非HAT区和人elp3 HAT区的融合yhelp3对上述缺陷有着更强的补偿能力。而HAT区催化结构域缺失的yhelp3HAT-没有任何补偿能力，表明人Elp3亚基可能与酵母的该亚基功能相似，人Elp3的HAT活性也为其行使功能所必需。